Immunization Strategies
Genovac’s core technology, genetic immunization, enables the development of antibodies against any protein target directly from DNA, an electronic sequence, or an accession number.
Genetic Immunization
Genovac’s core technology, genetic immunization, enables the development of antibodies against any protein target directly from nucleic acids, an electronic sequence, or an accession number.
In the case of DNA, Genovac clones your target into one of its proprietary immunization vectors, as detailed in your custom target analysis, and introduces it into the host organism for gene expression.
To help identify positive antisera, single B cells, and hybridomas, screening without purified antigen is made possible with the use of Genovac’s proprietary screening vectors. This technology has been applied to thousands of target proteins with a very high success rate.
Protein & Peptide Immunization
Protein immunization enables the generation of antibodies by directly injecting antigens into the host organism. In many cases, Genovac clients have access to purified recombinant proteins that can be used for immunization. If the antigen is not available, Genovac can express and purify your target in different systems (E. coli, insect, mammalian).
Peptide immunization enables the generation of antibodies by directly injecting peptide conjugates into the host organism. As part of the target analysis, Genovac can select linear epitopes as optimal candidates for immunization.
Genovac also offers peptide synthesis and conjugation services for both immunization and screening. Screening can be expanded to include, for example, phosphopeptide specificity for monoclonal development.
Protein and peptide immunizations are good options for bacterial targets. If a bacterial system is used to express the recombinant protein, then said protein will match the host bacterial protein post-translational modifications.
Other Immunization Options
In addition to our standard immunization technologies, a variety of other options are available, including cells, AAV, VLP, and nanodisc. Genovac offers a complimentary target analysis to determine which immunization strategy fits best for the clients’ prospective projects.