Polyclonal Antibodies Against Human RAS

POLYCLONAL ANTIBODIES AGAINST CYTOPLASMIC PROTEIN HUMAN RAS

THE CHALLENGE

Genovac was approached by a client who wanted polyclonal antibodies against the human protooncogene RAS GTPase in a rat host animal. However, this was a challenge since this intracellular target shares an extremely high amino acid sequence similarity with its rat orthologue, at 98%.


LEARNING MORE

The methods that were used in this project and described in this case study demonstrate the possibility to generate antibodies against highly conserved, intracellular proteins by genetic immunization. More info about the specific dilutions utilized to detect the RAS protein and view more data about our methodology and results can be found in our full case study, which can be found on our website.