Distinguishing Highly Conserved Molecules

THE CHALLENGE

Genovac was interested in running an internal project to demonstrate our capability in generating discriminating monoclonal antibodies against two tetraspanin proteins. Tetraspanins get their name from their four transmembrane domains, which are interspersed with short intra- and extracellular loops that form binding sites for other proteins. The generation of extracellular region binding antibodies against such multi-transmembrane spanning targets remains a challenge because they are highly conserved in their small extracellular loops.